DETERMINATION OF THROMBIN AND PLASMIN ACTIVITY IN HUMAN BLOOD PLASMA USING THE TURBIDIMETRIC CURVE OF CLOT FORMATION AND DISSOLUTION

The aim of the study was to develop a method for determination activity thrombin, which is based on turbidimetry curve formation and dissolution blood plasma clot. Methods. Donor samples were collected in 3.8% sodium citrate (1 part 9 parts blood, pH 7.4). Plasma separated from cells within 1 hour after collection by centrifugation latter at 1200 g 20 minutes. Aliquots stored -20 °C. Results. To determine concentrations thrombin plasmin, TDCs clots, initiated APTT reagent, used. Based values τ obtained, calibration constructed coordinates 1/τ – [Thr] (the rate protofibrils s-1 vs concentration NIH units ml). Conclusion. proposed methods plasmin made it possible quantitatively calculate prothrombin activation lag period, fibrin as well point complete clot dissolution,